Serum mixture.



appeared, injecting said EMIL Annnnnnnnnu, or irALLE-oN-rHE-sAALE, GERMANY.

SERUM. MIXTURE.

No lllrawingv To all whom itmay concern: I Be it known that I, EMIL doctor of medicine, professor of citizen of the German Empire, residing at Halle-on-the- Saale, Kingdom of Prussia, Germany, have invented new and useful Improvements in Serum Mixtures, of which the following is a'specification' This invention relates to methods for. determining the particular variety of cancer A D'ERHALDEN,

in an animal affected with, a tumor and.

further relates to new serum mixtures and their production.

More particularly it comprises of atumor carrier is biologically related to cancer of known variety, or to an antiserum which has been. produced by the known variety of tumor.

In my application Ser. No. 28,195 filed May 14, 1915, I have described new kinds of sera containing protective ferments which have an unusually powerful lysing action on cancerous tissue. These sera are obtained by injecting cancer albumin into an animal, Withdrawing serum therefrom before the protective ferments' first formed have'disserum into a fresh animal to produce a setum of augmented strength in protective ferments, and con tinuing the transference of serumthrough I a series of animals until a serum of the desired potency is secured. fThe potentiated serum so obtained has the valuable property that if injected into human beings suffering with carcinoma, for instance, it manifests distinct curative effects, as has been demonstrated by many clinical tests. This potentiated serum appears to have a specific destructive (lysing) action only on the-particular protein or protein derivative which originally induced its formation. Thus if cancer tissue or any other kind of tissue, or.

any peptone-mixture obtained from the protein of such tissues, is introduced par enterally e. subcutaneously or in intrave-' nously) into animals'or human beings, there appear in the serum of the animal or human being, protective ferments which have a se lective action upon the particular albumins or peptones introduced. Before using the potentiated serum in the treatment of cancer it is therefore necessary to make sure that the serum to be used corresponds to the particular variety of the albumin in the tumor to be treated. In devising a meth d physiology,

the tumor shows swelling and redness,

methods for ascertalning whether or not the cancer Specification of Letters Patent. P t t d J 13, 191 Application filed August 27, 1915. Serial Her-17,692. V I

for ascertaining whether or sary correspondence existed, theffollowing facts were taken into consideration.

If an animal with a cancerous tumor receives parenterally serum containing substances or protective ferments foreign to its blood but'corresponding'to said tumor, then r and cutaneous reactions (hyperem'ia and edema vu'lsions," fall of temperature, etc.,are observed. If however, this serum contains protective ferments, which. bear no relation to the var1 ety of the tumor carrier or'the tumor carr1er to be treated then no reactionv takes place.

The serum obtained from the test animals is called tumor antiserum, in contradistinction to the serum derived from the tumor carrier to be treated. Based on these obser- 'vations,'the following treatment of cancer has been developed.

Example 1.Determinwt i0n of the variety" of cancer of the tumor carrier to be treated-An animal is treated with cancerous tissue substances of a known variety. Into this animal the serum of the tumor not this necese5 'W1tl1. S11Ch general manifestations as coni carrier to be treated is introduced. It is then observed Whether manifestations as de-' scribed above develop. If this is the case, then the conclusion isjustified that both sera, namely the serum of the tumor carrier and thatof the previously treated animal, contain biologically related or identical products. Or the test animal is treated with a tumor anitserum and subsequently inoculated parenterally with the serum of the tumor carrier to be treated, and it is noted Whether a'reaction sets in or not.

Treatment of the tumor carrierr After it has been determined that the serum of the tumor carrier conforms to a certain antiserum, the tumor carrier to be treated is parenterally inoculated with the antiserum.

The properly selected antiserum manifests distinct curative effects in the tumor carrier. and complete disappearance of cancers of various types has been observed.

I' claim zv 1. A method for determining the correspondence of the protective ferments in a given antiserum with the cancer tissue of a tumor carrier,. comprising injecting said serum and the serum of said tumor carrier into a test animal to produce characteristic physiological disturbances if the constituanimal and then introducing serum vfrom the tumor carrier into the same animal, andr'e peating this operation with different cancertissue substances of known variety in different test animals until characteristic re-. actions occur,

such as convulsions and .fall

of temperature which indicate a correspondence, or blological relation; between the serum generated 1n the test animal and the serum from the tumor carrier, substantially as described. 'Intestimony whereof I have hereunto set my hand inthe presence of two subscribing Witnesses.

EMIL ABDERHALDEN. .Witnesses: a

' RU1 J0LBH Fnroxn;

. MARGARETE FRITZGHE; 

